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Plant-microbe interactions are critical to ecosystem resilience and substantially influence crop production. From the perspective of plant science, two important focus areas concerning plant-microbe interactions include: 1) understanding plant molecular mechanisms involved in plant-microbe interfaces and 2) engineering plants for increasing plant disease resistance or enhancing beneficial interactions with microbes to increase their resilience to biotic and abiotic stress conditions. Molecular biology and genetics approaches have been used to investigate the molecular mechanisms underlying plant responses to various beneficial and pathogenic microbes. While these approaches are valuable for elucidating the functions of individual genes and pathways, they fall short of unraveling the complex cross-talk across pathways or systems that plants employ to respond and adapt to environmental stresses. Also, genetic engineering of plants to increase disease resistance or enhance symbiosis with microbes has mainly been attempted or conducted through targeted manipulation of single genes/pathways of plants. Recent advancements in synthetic biology tool development are paving the way for multi-gene characterization and engineering in plants in relation to plant-microbe interactions. Here, we briefly summarize the current understanding of plant molecular pathways involved in plant interactions with beneficial and pathogenic microorganisms. Then, we highlight the progress in applying plant synthetic biology to elucidate the molecular basis of plant responses to microbes, enhance plant disease resistance, engineer synthetic symbiosis, and conduct in situ microbiome engineering. Lastly, we discuss the challenges, opportunities, and future directions for advancing plant-microbe interactions research using the capabilities of plant synthetic biology. 

Outbreaks of insects and diseases are part of the natural disturbance regime of all forests. However, introduced pathogens have had outsized impacts on many dominant forest tree species over the past century. Mitigating these impacts and restoring these species are dilemmas of the modern era. Here, we review the ecological and economic impact of introduced pathogens, focusing on examples in North America. We then synthesize the successes and challenges of past biotechnological approaches and discuss the integration of genomics and biotechnology to help mitigate the effects of past and future pathogen invasions. These questions are considered in the context of the transgenic American chestnut, which is the most comprehensive example to date of how biotechnological tools have been used to address the impacts of introduced pathogens on naïve forest ecosystems. 

For many lawmakers, energy-efficient buildings have been the main focus in large cities across the United States. Buildings consume the largest amount of energy and produce the highest amounts of greenhouse emissions. This is especially true for New York City (NYC)’s public and private buildings, which alone emit more than two-thirds of the city’s total greenhouse emissions. Therefore, improvements in building energy efficiency have become an essential target to reduce the amount of greenhouse gas emissions and fossil fuel consumption. NYC’s buildings’ historical energy consumption data was used in machine learning models to determine their ENERGY STAR scores for time series analysis and future pre- diction. Machine learning models were used to predict future energy use and answer the question of how to incorporate machine learning for effective decision-making to optimize energy usage within the largest buildings in a city. The results show that grouping buildings by property type, rather than by location, provides better predictions for ENERGY STAR scores. 

Target trial emulation is the process of mimicking target randomized trials using real-world data, where effective confounding control for unbiased treatment effect estimation remains a main challenge. Although various approaches have been proposed for this challenge, a systematic evaluation is still lacking. Here we emulated trials for thousands of medications from two large-scale real-world data warehouses, covering over 10 years of clinical records for over 170 million patients, aiming to identify new indications of approved drugs for Alzheimer’s disease. We assessed different propensity score models under the inverse probability of treatment weighting framework and suggested a model selection strategy for improved baseline covariate balancing. We also found that the deep learning-based propensity score model did not necessarily outperform logistic regression-based methods in covariate balancing. Finally, we highlighted five top-ranked drugs (pantoprazole, gabapentin, atorvastatin, fluticasone, and omeprazole) originally intended for other indications with potential benefits for Alzheimer’s patients. 

This paper presents a mobile-based solution that integrates 3D vision and voice interaction to assist people who are blind or have low vision to explore and interact with their surroundings. The key components of the system are the two 3D vision modules: the 3D object detection module integrates a deep-learning based 2D object detector with ARKit-based point cloud generation, and an interest direction recognition module integrates hand/finger recognition and ARKit-based 3D direction estimation. The integrated system consists of a voice interface, a task scheduler, and an instruction generator. The voice interface contains a customized user request mapping module that maps the user’s input voice into one of the four primary system operation modes (exploration, search, navigation, and settings adjustment). The task scheduler coordinates with two web services that host the two vision modules to allocate resources for computation based on the user request and network connectivity strength. Finally, the instruction generator computes the corresponding instructions based on the user request and results from the two vision modules. The system is capable of running in real time on mobile devices. We have shown preliminary experimental results on the performance of the voice to user request mapping module and the two vision modules. 

This paper proposes an AR-based real-time mobile system for assistive indoor navigation with target segmentation (ARMSAINTS) for both sighted and blind or low-vision (BLV) users to safely explore and navigate in an indoor environment. The solution comprises four major components: graph construction, hybrid modeling, real-time navigation and target segmentation. The system utilizes an automatic graph construction method to generate a graph from a 2D floorplan and the Delaunay triangulation-based localization method to provide precise localization with negligible error. The 3D obstacle detection method integrates the existing capability of AR with a 2D object detector and a semantic target segmentation model to detect and track 3D bounding boxes of obstacles and people to increase BLV safety and understanding when traveling in the indoor environment. The entire system does not require the installation and maintenance of expensive infrastructure, run in real-time on a smartphone, and can easily adapt to environmental changes.